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Synthesis and Anticoagulant activity of Sulfated alginate

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Waranyoo Pulsawat* and Suwannee Tongmalee

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Alginate is a natural polysaccharide containing carboxyl groups and (1-4) linkage between ?-D-mannuronic acid and ?-L-guluronic acid. Applying of sulfated modification for alginate could generate heparin-like structure containing sulfate, carboxyl group and uronic units. This research aimed to apply chemical modification using reaction between sodium alginate and sulfurochloridic acid (ClSO 3 H) in formamide to generate product possessing anticoagulant activity which was evaluated by activated partial thrombosis time (APTT) and prothrombin time (PT). The success of sulfation was determined by sulfate content measurement and verified by FTIR spectra. The alginate sulfates (AlgS) and alginate sulfate fragments (AlgHS) obtained containing sulfate content of 14.9 and 12.9 % with degree of sulfation (DS) of 1.75 and 1.35, respectively. The existence of sulfate groups was confirmed by two strong peaks at 797 and 1244 cm -1. The AlgS and AlgHS yielded APTT at approximately 287 and 102 seconds, respectively, at 75 g ml-1 but showed no increase of PT compared to the control assay. Sulfated alginate could be used for potential application such as anticoagulant.

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sulfated alginate, anticoagulant, APTT