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Studying Culture Conditions for High Phytase Production from Aspergillus fumigatus ET3

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Nguyen Van Tinh*, Nguyen Thi Ngoc Diep and Duong Thi Huong Giang

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This research aimed to isolate and study culture conditions for high level phytase biosynthesis of potential phytase producing Aspergillus fumigatus from rice soil samples in some provinces of Mekong Delta, Viet Nam. Among selected fungal isolates, the isolate ET3 exposed highest phytase activity on phytase screening medium. Based on morphological characteristics and sequences of 18S rRNA gene, ET3 appeared to belong to Aspergillus fumigatus species. The results also showed that the best conditions for high level phytase production from A. fumigatus ET3 on solid-state culture medium which contained wheat flour as a phytate substrate, was inoculated with 108 spores/ml of one-day-old spores, supplemented with 0.5% malt extract, 1% KH 2 PO 4 , 0.5% glucose and incubated for 2 days at pH 4, 35 C.

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Aspergillus fumigatus, extracellular phytase, phytate, 18S rRNA gene.