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Production of Resveratrol from Callus Cultures of Artocarpus lacucha Buch.-Ham.

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Narumon Bunchom, Methin Phadungkit, Weerachai Saijuntha, Pornthap Thanonkeo and Sudarat Thanonkeo

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Production of resveratrol from callus cultures ofArtocarpus lacucha Buch.-Ham. was investigated. Callus of A. lacucha was established and cultured at 25 ? 2 oC in Murashige and Skoog (MS) medium supplemented with various combinations of plant growth regulators, auxin and cytokinin. The accumulation of resveratrol in the cell cultures was determined by TLC densitometric method. The results revealed that callus cultures of A. lacucha produced and accumulated relatively high level of resveratrol. The A. lacucha callus cultures produced resveratrol simultaneously during the rapid growth phase of the culture cycle, suggesting that the production of resveratrol in this plant was growth-associated. Callus generated from shoots of A. lacucha produced and accumulated the highest yield of resveratrol (0.79 mgg-1DW) when cultured in MS medium supplemented with 4.5 ?M of 2, 4- dichlorophenoxy acetic acid (2,4-D) and 4.4 ?M of Benzylaminopurine (BAP), which was remarkably higher than that found in the heartwood of the intact plant.

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Artocarpus lacucha Buch.-Ham., resveratrol, callus culture, TLC densitometry