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Biogas Production from Hydrolysate Napier Grass by Co-Digestion with Slaughterhouse Wastewater using Anaerobic Mixed Cultures

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Sureewan Sittijunda

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Biogas production from co-digestion of hydrolyzed napier grass and slaughterhouse

wastewater using anaerobic mixed cultures was conducted. Factors influencing methane

production was investigated, i.e., initial pH (6, 7, 8) and carbon-nitrogen (C/N) ratio.

Optimum conditions were initial pH of 7 and C/N ratio of 3.42. Under these conditions,

a methane production (MP), methane production rate (MPR) and methane yield (MY) of

299.69 ml CH4/L, 0.52 ml CH4/L h, and 39.76 ml CH4/g-COD were obtained. Using the

optimal conditions, MP, MPR and MY from co-digestion of hydrolyzed napier grass and

slaughterhouse wastewater (299.69 ml CH4/L, 0.52 ml CH4/L h and 39.76 ml CH4/g-COD)

were 1.82, 1.79 and 2.11 times greater than that of the controls (without inoculum or self

fermentation) (164.63 mL-CH4/L, 0.29 mL-CH4/L h and 18.76 ml CH4/g-COD). The

energy production from co-digestion of hydrolyzed napier grass and slaughterhouse

wastewater was 11.99 kJ/L.

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methane production; hydrolysate napier grass; slaughterhouse wastewater