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Application of Microsatellite Markers for Identification of Wine Grape Varieties in Thailand

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Alongkorn Sripholtaen, Charoen Charoenchai and Hathairat Urairong

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The use of Microsatellite markers for identifying grapevine varieties. Eighteen

primer pairs were selected for identifying amplify 29 grapevine varieties. The result

shower that a total of 133 alleles with an average of 7.4 alleles per locus were found to be

polymorphic. All alleles size were approximately 112-262 base pair. The cluster analysis

using unweighted pair group method with arithmetic mean (UPGMA) based on genetic

dissimilarity matrix with 0.9. Twenty nine grapevine varieties were clustered into two

major groups. This research indicated that the microsatellite markers could be used to

examine and compare the genetic resources among closely samples. The microsatellite

maker is useful for discrimination and analysis of genetic diversity of grapevine varieties,

and also for grapevine breeding in the future.

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Grapevine; DNA fingerprint; Microsatellite marker